eng
Tissues and Biomaterial Research Group (TBRG)-USERN
Journal of Tissues and Materials
2645-3487
2676-6981
2019-09-01
2
3
1
8
10.22034/jtm.2019.199830.1022
93086
مقاله پژوهشی
Crystallographic study of hydrothermal synthesis of Hydroxyapatite nano-rods using Brushite precursors
Hassan Nosrati
h.nosrati@modares.ac.ir
1
Rasoul Sarraf-Mamoory
rsarrafm@modares.ac.ir
2
Fatemeh Dabir
fdabir@nri.ac.ir
3
Department of Materials Engineering, Tarbiat Modares University, Tehran, Iran
Department of Materials Engineering, Tarbiat Modares University, Tehran, Iran
Non-metallic Materials Group, Niroo Research Institute, Tehran, Iran
Introduction: Being known for an array of properties that favor hard tissue regeneration, ranging from osteoconductivity to biocompatibility to non-immunogenicity, and being the natural bone mineral phase hydroxyapatite (HA, Ca10(PO4)6(OH)2) is the natural bioceramic of choice for the reinforcement phase of biocomposites. Objective: The main objective of this study is to successfully synthesize uniform one dimensional HA nano-structures using a gram-scale hydrothermal batch process. Material and Methods: Brushite used as a precursor for HA synthesis. The powders obtained after washing and drying were evaluated. The analysis performed in the sample includes inductively coupled plasma (ICP), Raman Spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, Field Emission Scanning Electron Microscope (FE-SEM), and high-resolution TEM. Result: The results of this study showed that the initial brushite used in the hydrothermal process was dissolved, followed by the nucleation process and the growth of hydroxyapatite. The synthesized powders in this study were rod-shaped, with 35 nm in diameter and between 50 and 250 nm in length. The main direction of rod growth was , which is C axis. Conclusion: The powders synthesized in this research have the potential to be used in bone tissue engineering, implantation, and drug delivery.
https://www.jourtm.com/article_93086_e1fb2881dedc8fbdd3856b898d87e26f.pdf
Hard Tissue Engineering
Hydroxyapatite
Nano-rods
Hydrothermal
eng
Tissues and Biomaterial Research Group (TBRG)-USERN
Journal of Tissues and Materials
2645-3487
2676-6981
2019-09-01
2
3
9
17
10.22034/jtm.2019.199970.1023
93087
مقاله پژوهشی
Fabrication of polycaprolactone scaffold with gradient porous microstructure for bone tissue engineering
Mahdokht Akbari Taemeh
mahdokht.akbari@alumni.ut.ac.ir
1
Babak Akbari
babakbari@ut.ac.ir
2
Mojtaba Khorramnezhad
m.khorramnezhad@alumni.ut.ac.ir
3
Jhamak nourmohammadi
j_nourmohammadi@ut.ac.ir
4
Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran
Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran
Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran
Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran
Introduction: Selective laser sintering, electrospinning, Layer by Layer Assembly, porogen leaching and additive manufacturing are applied methods in fabrication of gradient scaffolds with limitations such as being expensive or complicated. Objective: The main purpose of this study was to apply a novel and simple method in fabrication of gradient scaffolds with minimum cost. Methods: Two types of homogenous and two types of gradient scaffolds were fabricated by combining layer-by-layer assembly and porogen leaching techniques in a new manner. Pore size gradient was created along the radial direction by using paraffin micro particles as porogen and two different size of syringe as mold. The first layer was made in the smaller mold, with a specific size range of porogen and the second layer was fabricated around the inner one using porogens with a different size range from the first layer. Results: Scanning electron microscope images of scaffolds showed spherical pores and the structure of gradient scaffolds showed the radial gradient with a good adhesion between layers without any detectable interface. The porosity of scaffolds was 77.5 ± 3 % and 61.3 ± 4 % for homogenous and 74 ± 2.8 % and 79.8 ± 2.3 % for gradient scaffolds which are suitable for bone tissue engineering. Mechanical properties of scaffolds were better for lower porosities. The results indicated that gradient porous structure had no considerable effect on mechanical properties. MTT assay and cell morphology tests showed scaffolds biocompatibility. Conclusion: The applied method is suitable for pore size gradient creation. Gradient scaffolds can be used to investigate the influence of pore size gradient on biologic properties, cells differentiation and cell distribution and bone formation.
https://www.jourtm.com/article_93087_ef412abc4012f704a406a869602f8068.pdf
polycaprolactone
pore size gradient scaffold
radial gradient
Layer by layer assembly
porogen leaching
eng
Tissues and Biomaterial Research Group (TBRG)-USERN
Journal of Tissues and Materials
2645-3487
2676-6981
2019-10-29
2
3
18
30
10.22034/jtm.2019.93448
93448
مقاله پژوهشی
An antibacterial delivery system based on Eucalyptus camaldulensis loaded in starch microspheres
Leila Sadeghzadeh
1
Mohammad Rafienia
2
Fatemeh Sefidkon
3
Parviz Owlia
4
Babak Negahdari
5
Shahin Bonakdar
shahinbonakdar@yahoo.com
6
Nanotechnology Researchers Company, Tehran, Iran.
Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Research Institute of Forests and Rangelands, Tehran, Iran.
Molecular Microbiology Research Center, Shahed University, Tehran, Iran.
School of Advanced Technologies in Medicine, Tehran University of Medical Science. Iran.
National Cell Bank Department, Pasteur Institute of Iran, Tehran, Iran.
Eucalyptus camaldulensis oil (EO) loaded microspheres were prepared by water-in-oil emulsification cross-linking reaction method using starch as raw material. EO as a natural drug was extracted from Eucalyptus camaldulensis leaves. The effects of mechanical stirrer rate and time of cross-linking on antimicrobial behavior (against Micrococcus luteus and Escherichia coli), morphology and particle size of EO-loaded microspheres were investigated. These microspheres were characterized by FTIR spectroscopy and laser light scattering. Microscopic observations by SEM revealed the spherical and smooth surfaces of the microspheres. Investigations showed that the mean particle size of microspheres decreased from 11.34 to 9.45 µm while the homogenizer speed increased from 8500 to 13500 rpm. Moreover, the microspheres were effective in releasing EO over an extended period of about 100 h in phosphate buffer saline (pH 7.4) and all of the formulations showed antibacterial activity, while this behavior was more noticeable on E. coli. In conclusion, starch microspheres can be used as drug delivery vehicles to sustain the release of antibiotics to prevent infections associated with medical devices.
https://www.jourtm.com/article_93448_d41d8cd98f00b204e9800998ecf8427e.pdf
antibacterial
Drug Delivery
Eucalyptus oil
Microspheres
Starch