Comparative analysis of gelatin and hydroxyethyl cellulose scaffolds crosslinked by silane coupling agent

Document Type : Original Article

Authors

1 Department of Nanotechnology and Advanced Materials Research, Materials & Energy Research Center, Karaj, Iran

2 Department of Nanotechnology and Advanced Materials Research, Materials & Energy Research Center, Karaj, Iran.

3 Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

10.22034/jtm.2020.249653.1031

Abstract

The purpose of this study was to fabricate gelatin (G) and hydroxyethyl cellulose (HEC) scaffolds as promising for skin tissue engineering. Therefore, crosslinking is essential to prepare the mentioned scaffolds. ɣ -Glycidoxypropyltrimethoxysilane (GPTMS) was added to polymeric solutions as a cross-linker agent and then freeze-dried. Scanning electron microscopy (SEM) micrographs indicated that the average pore size of the G-GPTMS and HEC-GPTMS was 54 and 120 µm, respectively. According to the dynamic rheology measurements (DMA), both solutions were flowable in which, the loss modulus was higher than the storage one in HEC-GPTMS and G-GPTMS did not have storage modulus. These loose structures might be depending on the formation of water molecules during sol-gel reaction. Moreover, HEC-GPTMS revealed shear thinning behavior and G-GPTMS showed shear thickening behavior. The G-GPTMS sample showed lower swelling ratio and degradation degrees as compared to HEC-GPTMS scaffold. But due to hydrophilicity and macroporous structures, both scaffolds showed 1700% swelling ratio.

Keywords