Document Type: Original Article
Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
Faculty of chemistry and chemical Engineering, Malek Ashtar university of technology, Tehran, Iran
Faculty of Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, Iran
National Cell Bank Department, Pasteur Institute of Iran, Tehran, Iran
The controlled release drug delivery preserves the efficient concentration of drug in blood and omits the side effect of it. Also, it provides more comfort and high quality of life for patients. Polymers, one of the common substrate of drug delivery systems, usually were used in nano-particle shape, that are one of the appropriate methods for controlled and efficient drug delivery. Among the methods for preparation of nano-particles, electrospray because of its simple control on the rate of deposition, size, and density of particles, low-cost equipment, fabrication in atmospheric condition and low temperature is a unique way.
In this study, the theophylline(TF) was encapsulated in PVA by electrospray for controlled release drug delivery purposes. For determining the optimized particle, the first different molecular weights of PVA (23000, 72000 and 144000 gr/mol) were assessed by SEM pictures. Then different concentrations of PVA (5, 10, 13 and 15% w/v) were evaluated by SEM for the best homogenous particles. Afterward, the appropriate method for crosslinking of particles (glutaraldehyde for 24&48 h and oven in 130 ºC) was determined. Finally, theophylline was added to the PVA solution and encapsulated by electro-spraying and morphology of particles was investigated by rhodamine staining and SEM.
SEM results of the different molecular weight of electro-sprayed PVA particles were determined in the sample with 23000 g/mol (98% hydrolyzed) was the best one. For choosing of the optimized concentration of PVA, SEM, and rhodamine staining results showed that particles with 10% w/v PVA were the most homogenous ones. After encapsulation of 5% w/v TF in PVA, the morphology of particles after crosslinking was determined the oven in 130 ºC and glutaraldehyde for 24h was the appropriate method for crosslinking of particles because of the stable and homogenous morphology of them. So PVA nano-particles have shown as appropriate and promising systems for sustained drug delivery.