Decellularized lung tissue enhanced mesenchymal stem cells proliferation and differentiationn

Document Type: Original Article


1 Faculty of Biomedical Engineering, Islamic Azad University Tehran Central Branch, Tehran, Iran

2 Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

3 Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran



At Decellularized lung tissue enhanced mesenchymal stem cells proliferation and differentiation. The combination of mesenchymal stem cells (MSCs) isolated from rat bone marrow and extracellular matrix (ECM)- derived scaffolds present a promising approach in tissue engineering and regenerative medicine. extracellular matrix physically supports stem cells niche and influence stem cell proliferation and differentiation. In this study, we investigated the effects of decellularized lung tissue (DLT), which was decellularized by SDS and triton x-100 and Dnase1, on proliferation and chondrogenic properties of mesenchymal stem cells. Hematoxyline - Eozin (H&E) staining and DNAcontent and SEM confrimed the cell in the accellularized lung tissue. MTT assay showed that decellularized lung tissue enhanced the cell viability of mesenchymal stem cells. Alcian blue and immunostaining confirmed that decellularized lung tissue ameliorate the chondrogenesis when compared with tissue culture plate. Decellularization of lung tissue seems to be a good tool to proliferation and differentiation of mesenchymal stem cells.

Graphical Abstract

Decellularized lung tissue enhanced mesenchymal stem cells proliferation and differentiationn


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