@article { author = {Samani, Sanaz and Shokrgozar, Mohammad Ali and Zamini, Arash and Majidi, Mohammad and Tavassoli, Hossein and Aidun, Amir and Bonakdar, Shahin}, title = {Preparation of Skin Tissue Engineering Scaffold Based on Adipose-Derived Tissue}, journal = {Journal of Tissues and Materials}, volume = {2}, number = {1}, pages = {47-54}, year = {2019}, publisher = {Tissues and Biomaterial Research Group (TBRG)-USERN}, issn = {2645-3487}, eissn = {2676-6981}, doi = {10.22034/jtm.2019.83230}, abstract = {Introduction: In clinical tissue engineering, scaffolds play a critical role in the formation of the appropriate physical, chemical and biological environment. It seems that extracellular matrix-based materials such as adipose supports the cellular functions like adhesion, growth or differentiation. Objective: In this study, human-derived adipose tissue was modified with different chemically crosslinking methods including carbodiimide, aldehyde and isocyanate reagents to fabricate skin tissue engineered scaffolds. Material and Methods: In this study, biological-based scaffolds were fabricated through modification of liposuction tissues and crosslinked by different chemical agents including glutaraldehyde (GLA), hexamethylene diisocyanate (HMDI) and 1-Ethyl-3-3-dimethylaminopropyl carbodiimide (EDC). The chemically formed bands were characterized by Fourier Transform Infrared spectroscopy (FTIR) and mechanical properties analyzed by standard compression testing. Adipose-derived mesenchymal stem cells (ADSCs) were isolated and cultured on the samples. Result: The MTT assay and microscopy observations of cultured adipose-derived stem cells confirmed the biological performance of these scaffolds in vitro.  The degradation results on phosphate buffer saline also showed that crosslinking with 10 mM concentration of EDC preserve the scaffold integrity for 2 months. Real-time PCR observations confirmed that ADSCs were  Conclusion: According to the results, adipose-derived scaffolds could find broad clinical applications in tissue engineering for skin regenerations in deep burns or plastic surgeries.}, keywords = {Skin tissue engineering,Adipose-derived stem cells,Human adipose tissue,Extra-cellular matrix}, url = {https://www.jourtm.com/article_83230.html}, eprint = {https://www.jourtm.com/article_83230_321ca32d02a88962ef8b56c5fb6ddd2e.pdf} }