TY - JOUR ID - 84397 TI - Improved Biomedical Properties of Chitosan/Alginate Composites by Chemical Immobilization of Gelatin layer JO - Journal of Tissues and Materials JA - JTM LA - en SN - 2645-3487 AU - Haghjoo, Roghayeh AU - Karkhaneh, Akbar AD - biomaterial, biomedical engineering, science and research branch of islamic azad university, tehran, iran AD - Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran Y1 - 2019 PY - 2019 VL - 2 IS - 1 SP - 14 EP - 22 KW - Gelatin KW - alginate KW - Chitosan KW - Glutaraldehyde KW - Immobilization KW - Surface modification DO - 10.22034/jtm.2019.174222.1015 N2 - Introduction: Recently, creating bioactive wound dressings from natural polymers is of importance.  Also, there is high need for a device to staunch blood flow in deep wounds such as liver and spleen wounds, and would absorb in the body on its own. This device must be extremely safe, act fast, and adjust with local and general body health. Objective: This project designed surface modification for films made from natural Chitosan-Alginate polymers containing multiple ratios via chemically immobilizing the gelatin polymer. Material and methods: The scaffolds were synthesized by electrospinning method. In this regard, polymers were dissolved in the solvent and then graphene oxide was added into polymeric solution with a ratio of 2% and 4%. The parameters of the scaffold evaluated via scanning electron microscopes (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray Diffraction (XRD), contact angle, alizarin red stating, and alkaline phosphatase (ALP). For evaluation of the cell behavior on the scaffolds, the MG-63 was used. Result: Chitosan-Alginate (C-A) solutions were prepared in 8:2, 7:3 and 5:5 weight percentages. Afterwards, these solutions were used to make composite films to use as solvent casting. Gelatin (G) was immobilized onto C-A films using the crosslinking reagents, which included Glutaraldehyde. The effects of C-A ratio, gelatin concentration, amount of crosslinking agent and duration of immobilization process on the actual immobilized layer were investigated. The films were characterized by Scanning Electron Microscopy (SEM), Furrier Transformer Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) and Zeta Potential. SEM images demonstrated that immobilization onto 7:3 and 5:5 weight percentages ratios of C-A, resulted in a more consistent gelatin layer compared to 8:2. FTIR stereoscopy, which showed the appearance of amid peak in modified films. In DSC curves, suppression in Glass Transmission Temperature (Tg) in modified films was detected. Also, Zeta potential decreased as the amount of gelatin layer on C-A films was increased. Conclusion: The newly developed (C-A-G) Composite films by simple, yet effective method of immobilization can be used for various biomedical applications like tissue engineering and wound healing. UR - https://www.jourtm.com/article_84397.html L1 - https://www.jourtm.com/article_84397_cb60532c3c12f1f8c382f25f856191a2.pdf ER -