Preparation and Evaluation of Chitosan-Carboxymethyl Cellulose Composite Film with Menta longifolia Extract for Wound Healing Activity

Document Type : Original Article


1 Department of Biomedical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Biomedical Engineering, Faculty of engineering, Maziar University, Noor, Iran

3 Department of Biomedical Engineering, Tehran center Branch, Islamic Azad University, Tehran, Tehran, Iran

4 Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran



The aim of this study was to investigate the antibacterial and antifungal properties of Menta longifolia extract on chitosan-carboxymethyl cellulose composite bed for wound making. For this purpose, the effect of the ethanol extract of Oregano plant on the composite film bed of chitosan-carboxymethyl cellulose was investigated. Concentrations of 5%, 10%, 20% ethanolic extracts of oregano leaves with control sample (pure film) were investigated. The results of the experiments and the tests and another showed that Pune extract has antimicrobial properties and is resistant to gram-positive bacteria in the presence of 20% of the extract on the film substrate, more than the sample with concentration Lower extract and pure chitosan-carboxymethyl cellulose film. Also, the presence of Menta longifolia extract with 40% concentration increased the water absorption rate and inflation rate. Surface morphology also showed that increasing the concentration of extract in the film can improve the healing activity of the wound.