Decellularized lung tissue enhanced mesenchymal stem cells proliferation and differentiationn

Document Type : Original Article


1 Faculty of Biomedical Engineering, Islamic Azad University Tehran Central Branch, Tehran, Iran

2 Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

3 Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran



At Decellularized lung tissue enhanced mesenchymal stem cells proliferation and differentiation. The combination of mesenchymal stem cells (MSCs) isolated from rat bone marrow and extracellular matrix (ECM)- derived scaffolds present a promising approach in tissue engineering and regenerative medicine. extracellular matrix physically supports stem cells niche and influence stem cell proliferation and differentiation. In this study, we investigated the effects of decellularized lung tissue (DLT), which was decellularized by SDS and triton x-100 and Dnase1, on proliferation and chondrogenic properties of mesenchymal stem cells. Hematoxyline - Eozin (H&E) staining and DNAcontent and SEM confrimed the cell in the accellularized lung tissue. MTT assay showed that decellularized lung tissue enhanced the cell viability of mesenchymal stem cells. Alcian blue and immunostaining confirmed that decellularized lung tissue ameliorate the chondrogenesis when compared with tissue culture plate. Decellularization of lung tissue seems to be a good tool to proliferation and differentiation of mesenchymal stem cells.

Graphical Abstract

Decellularized lung tissue enhanced mesenchymal stem cells proliferation and differentiationn


[1]          Hollister SJJNm. Porous scaffold design for tissue engineering. 2005;4(7):518.
[2]          Ayala R, Zhang C, Yang D, Hwang Y, Aung A, Shroff SS, et al. Engineering the cell–material interface for controlling stem cell adhesion, migration, and differentiation. 2011;32(15):3700-11.
[3]          Patel M, Fisher JPJPr. Biomaterial scaffolds in pediatric tissue engineering. 2008;63(5):497.
[4]          Crapo PM, Gilbert TW, Badylak SFJB. An overview of tissue and whole organ decellularization processes. 2011;32(12):3233-43.
[5]          Ross EA, Williams MJ, Hamazaki T, Terada N, Clapp WL, Adin C, et al. Embryonic stem cells proliferate and differentiate when seeded into kidney scaffolds. 2009;20(11):2338-47.
[6]          Greenwald S, Berry CJTJop. Improving vascular grafts: the importance of mechanical and haemodynamic properties. 2000;190(3):292-9.
[7]          Mendez JJ, Ghaedi M, Steinbacher D, Niklason LEJTEPA. Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds. 2014;20(11-12):1735-46.
[8]          Young BM, Shankar K, Allen BP, Pouliot RA, Schneck MB, Mikhaiel NS, et al. Electrospun Decellularized Lung Matrix Scaffold for Airway Smooth Muscle Culture. 2017;3(12):3480-92.
[9]          Nemati S, Masudi NSJJoBR. Comparative Scale-Up Culture of Human Neural Progenitor Cells Derived from Human Embryonic Stem Cell and Transdifferentiated Human Foreskin Fibroblast under Defined Condition in Stirred Bioreactor. 2019;1(1):1-15.
[10]       Köchl S, Niederstätter H, Parson W. DNA extraction and quantitation of forensic samples using the phenol-chloroform method and real-time PCR.  Forensic DNA typing protocols: Springer; 2005. p. 13-29.
[11]       Rahimi A, Amiri I, Roushandeh AM, Choshali ZG, Alizadeh Z, Artimani T, et al. Sublethal concentration of H 2 O 2 enhances the protective effect of mesenchymal stem cells in rat model of spinal cord injury. 2018;40(3):609-15.
[12]       Gilbert TW, Sellaro TL, Badylak SFJB. Decellularization of tissues and organs. 2006;27(19):3675-83.
[13]       Kuttan R, Spall R, Duhamel R, Sipes I, Meezan E, Brendel KJL. Preparation and composition of alveolar extracellular matrix and incorporated basement membrane. 1981;159(1):333-45.
[14]       Nia T, Alizadeh A, Takhshid MA, Sadroddiny EJJoATE. Decellularization of lung tissue and analysis of its differentiative potential on bone marrow mesenchymal stem cells of rat. 2015;2(1).
[15]       Vavken P, Joshi S, Murray MMJJoor. TRITONā€X is most effective among three decellularization agents for ACL tissue engineering. 2009;27(12):1612-8.
[16]       Flynn LJB. The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells. 2010;31(17):4715-24.
[17]       Schlie-Wolter S, Ngezahayo A, Chichkov BNJEcr. The selective role of ECM components on cell adhesion, morphology, proliferation and communication in vitro. 2013;319(10):1553-61.
[18]       Nam J, Huang Y, Agarwal S, Lannutti JJTe. Improved cellular infiltration in electrospun fiber via engineered porosity. 2007;13(9):2249-57.
[19]       Wei W, Li J, Chen S, Chen M, Xie Q, Sun H, et al. In vitro osteogenic induction of bone marrow mesenchymal stem cells with a decellularized matrix derived from human adipose stem cells and in vivo implantation for bone regeneration. 2017;5(13):2468-82.
[20]       Ji J, Zhang D, Wei W, Shen B, Zhang Y, Wang Y, et al. Decellularized matrix of adipose-derived mesenchymal stromal cells enhanced retinal progenitor cell proliferation via the Akt/Erk pathway and neuronal differentiation. 2018;20(1):74-86.
[21]       Bombelli S, Meregalli C, Scalia C, Bovo G, Torsello B, De Marco S, et al. Nephrosphere-derived cells are induced to multilineage differentiation when cultured on human decellularized kidney scaffolds. 2018;188(1):184-95.
[22]       Ahani E, Montazer M, Toliyat T, Mahmoudi Rad M. A novel biocompatible antibacterial product: Nanoliposomes loaded with poly(hexamethylene biguanide chloride). Journal of Bioactive and Compatible Polymers. 2017;32(3):242-262.
[23]       Ahani E, Montazer M, Toliyat T, Mahmoudi Rad M. Preparation of nano cationic liposome as carriermembrane for polyhexamethylene biguanide chloridethrough various methods utilizing higher antibacterialactivities with low cell toxicity. Journal of microencapsulation.2017;34(2);121-131.
[24]       Caralt M, Uzarski JS, Iacob S, Obergfell KP, Berg N, Bijonowski BM, et al. Optimization and critical evaluation of decellularization strategies to develop renal extracellular matrix scaffolds as biological templates for organ engineering and transplantation. 2015;15(1):64-75.